WGS and Metagenomics
At the forefront of genomic research, our Whole Genome Sequencing (WGS) service harnesses the power of the Revio PacBio platform. Offering unmatched accuracy, long-read sequencing, and comprehensive genome coverage, this service is designed for diverse applications, from human genomics to complex plant and microbial research.
Why Choose Us?
- Expertise: Years of experience in genomics and bioinformatics.
- Tailored Solutions: Customized project designs to meet diverse research goals.
- Advanced Technology: State-of-the-art Revio PacBio systems for superior data quality.
- End-to-End Support: From experimental design to data analysis, we’re with you every step of the way.
Key Features of Revio PacBio WGS
Unparalleled Accuracy:
Powered by HiFi reads, the Revio PacBio system delivers sequencing results with >99.9% accuracy, ideal for resolving errors in repetitive and GC-rich regions.
Long-Read Capability:
With reads averaging 15–20 kb or longer, the platform excels in resolving structural variations and assembling genomes de novo.
De Novo Assembly Excellence:
The Revio PacBio system provides high-quality, contiguous genome assemblies, even for large, complex genomes or those with repetitive regions.
Scalable Throughput:
Efficient processing for large projects, including population studies and metagenomics analyses.
Comprehensive Data Outputs:
High-quality sequence data for genome assemblies, structural variant analysis, epigenetics (e.g., methylation), and more.
Applications of WGS on Revio PacBio
Human Genomics
Benefits: Detection of rare variants, structural variations, and expanded genomic regions.
Example Use Cases: Inherited disease studies, cancer genomics, pharmacogenomics.
Plant Genomics
Benefits: Resolving complex genomes with polyploidy and high heterozygosity.
Example Use Cases: Crop improvement, trait discovery, and stress tolerance research.
Animal Genomics
Benefits: Structural variant detection and high-quality genome assemblies for livestock and wildlife.
Example Use Cases: Animal breeding, conservation genomics, and health research.
Microbial Genomics
Benefits: Complete microbial genome assembly and diversity analysis.
Example Use Cases: Pathogen characterization, strain typing, and antibiotic resistance studies.
Metagenomics
Benefits: High-resolution taxonomic profiling and functional analysis of mixed microbial communities.
Example Use Cases: Gut microbiome research, environmental studies, and industrial microbiology.
De Novo Assembly
Benefits: Construction of highly contiguous, reference-grade genome assemblies.
Example Use Cases: Assembling genomes of novel species, improving fragmented assemblies, and evolutionary studies.
Applications of WGS on Revio PacBic:
Effective DNA QC is critical for ensuring high-quality sequencing data. Below is a comparison of DNA QC requirements for plants, animals, microbes, and metagenomics projects:
Plants
Required DNA Amount: 5–10 µg
Purity (A260/A280): 1.8–2.0
Integrity: High-molecular-weight DNA (>20 kb); minimal degradation.
Key Challenges: High polysaccharide and phenolic contamination.
Animals
Required DNA Amount: 2–5 µg
Purity (A260/A280): 1.8–2.0
Integrity: Intact DNA; minimal fragmentation.
Key Challenges: Tissue-specific variations; some tissues may yield degraded DNA.
Microbes
Required DNA Amount: 1–2 µg
Purity (A260/A280): 1.8–2.0
Integrity: High-molecular-weight DNA (>15 kb) for accurate assemblies.
Key Challenges: Extraction challenges with tough cell walls (e.g., Gram-positive bacteria).
Metagenomics
Required DNA Amount: 5–10 ng (per organism)
Purity (A260/A280): 1.8–2.0
Integrity: Even representation of DNA from mixed samples; high integrity.
Key Challenges: Low-abundance DNA, contamination by host DNA, and bias during extraction.
De Novo Assembly
Required DNA Amount: 5–20 µg (depending on genome size)
Purity (A260/A280): 1.8–2.0
Integrity: Ultra-high-molecular-weight DNA (>50 kb preferred).
Key Challenges: Handling large, complex genomes with repetitive regions and high heterozygosity.
De Novo Genome Assembly: The Gold Standard
De novo genome assembly reconstructs the genome from scratch without relying on a reference sequence. This is especially critical for species without existing reference genomes or for improving fragmented assemblies.
Advantages of De Novo Assembly with Revio PacBio:
- Contiguity: Generate highly contiguous assemblies with minimal gaps.
- Resolution: Resolve complex regions, including repetitive elements, structural variations, and telomeric regions.
- Flexibility: Suitable for genomes of all sizes, from small microbial genomes to large polyploid plant genomes.
- Epigenetic Insight: Capture epigenetic modifications (e.g., methylation) directly from native DNA.
Workflow Overview
Sample Preparation & QC
Thorough evaluation of DNA integrity, concentration, and purity to ensure optimal sequencing results.
Library Construction
Preparation of high-quality PacBio libraries tailored for HiFi sequencing.
Sequencing
High-throughput sequencing on the Revio PacBio platform.
Data Analysis
Comprehensive bioinformatics pipeline, including:
- Genome assembly
- Variant detection (SNPs, SVs)
- Functional annotation
- Metagenomic profiling (for mixed samples)
Get Started Today
Contact us to learn how our WGS services can advance your research. Our team is ready to provide a tailored solution that meets your needs, whether you’re investigating human disease, advancing agricultural genomics, or exploring microbial diversity.
Gene Omics s.r.o.
Republikánská 1218/6, Lobzy, 312 00 Plzeň
IČ: 22273310
Contacts
info@geneomics.eu
+420 725 137 589
