MAS-Seq
Our MAS-Seq (Multiplexed Amplicon Sequencing) RNA Sequencing service combines the power of the Revio PacBio platform with specialized protocols to deliver precise, full-length RNA sequencing for multiplexed samples.
This service is designed to capture and analyze complex transcriptomes efficiently, providing insights into isoform diversity, gene expression, and RNA modifications.
MAS-Seq RNA sequencing offers unmatched accuracy through HiFi reads, delivering highly reliable data for a wide range of research applications, from functional genomics to transcriptome profiling in various organisms.
Advantages of MAS-Seq RNA Sequencing
- Unparalleled Accuracy: HiFi sequencing ensures high confidence in transcript identification and quantification.
- Efficient Multiplexing: Analyze multiple samples in a single run, reducing costs and turnaround times.
- Full-Length Coverage: Capture entire RNA molecules for comprehensive transcriptomic insights.
- Flexible Applications: Suitable for diverse research fields, including functional genomics, epigenetics, and disease studies.
- Comprehensive Support: From sample QC to advanced bioinformatics, our team provides end-to-end assistance for your project.
Key Features of MAS-Seq RNA Sequencing
Full-Length Transcript Sequencing:
Capture entire RNA molecules, preserving native structure and sequence.
High Throughput with Multiplexing:
Process multiple samples in parallel without compromising data quality.
HiFi Accuracy:
Achieve >99.9% accuracy, ensuring precise isoform identification and quantification.
Low Input Requirements:
Suitable for projects with limited RNA availability or challenging sample types.
Cost-Effective Solutions:
Efficient multiplexing reduces per-sample costs while maintaining high-quality results.
Applications of MAS-Seq RNA Sequencing
Isoform Discovery
Benefits: Comprehensive detection of splice isoforms across multiplexed samples.
Example Use Cases: Annotation of novel isoforms in plants, animals, or microbes.
Gene Expression Profiling
Benefits: Quantitative and qualitative insights into transcriptomes.
Example Use Cases: Comparative studies across conditions, tissues, or species.
Alternative Splicing Analysis
Benefits: Detection and quantification of splicing variants.
Example Use Cases: Functional studies on alternative splicing in disease models.
Epigenetics & Modifications
Benefits: Detection of RNA modifications like m6A, m5C, and pseudouridine.
Example Use Cases: Regulatory RNA modification studies in stress responses or developmental processes.
Functional Genomics
Benefits: Integration of transcriptomic data into functional annotations.
Example Use Cases: Gene ontology and pathway enrichment studies.
RNA Input Requirements for MAS-Seq RNA Sequencing
To ensure optimal sequencing results, RNA quality and concentration must meet the following requirements:
Short Transcripts
Target Transcript Length: <1 kb
Required Input Amount: ≥ 50 ng RNA
Purity (RIN): ≥ 7
Recommended QC Metrics: Clear peaks on electropherogram with minimal degradation.
Notes: Suitable for small mRNAs and non-coding RNAs.
Medium Transcripts
Target Transcript Length: 1–5 kb
Required Input Amount: ≥ 100 ng RNA
Purity (RIN): ≥ 8
Recommended QC Metrics: Intact RNA with high 28S/18S ratio.
Notes: Ideal for standard transcriptomics and gene expression studies.
Long Transcripts
Target Transcript Length: >5 kb
Required Input Amount: ≥ 200 ng RNA
Purity (RIN): ≥ 8
Recommended QC Metrics: High-molecular-weight RNA preferred.
Notes: Required for long non-coding RNAs or extended UTR regions.
Multiplexed Samples
Target Transcript Length: Variable
Required Input Amount: ≥ 10 ng RNA/sample
Purity (RIN): ≥ 7
Recommended QC Metrics: Equal RNA input for each sample in the pool.
Notes: Designed for projects requiring parallel analysis of multiple samples.
Workflow Overview
Sample Preparation & QC
- Multiplexed samples are pooled in equal proportions to ensure balanced sequencing.
cDNA Synthesis
- Reverse-transcription of RNA into full-length cDNA using MAS-Seq optimized protocols.
Library Preparation
- HiFi libraries are constructed with barcoding for multiplexed analysis, ensuring high accuracy for each sample.
Revio PacBio Sequencing
- Highly accurate long-read sequencing captures full-length RNA molecules for each sample in the pool.
Data Analysis
- Isoform Analysis: Comprehensive identification and quantification of splice isoforms.
- Gene Expression Profiling: Differential expression analysis across samples.
- RNA Modifications: Detection and annotation of RNA modifications.
- Functional Annotations: Integration with gene ontology and pathway databases.
Get Started with MAS-Seq RNA Sequencing
Our MAS-Seq RNA Sequencing service delivers accurate, high-throughput transcriptomic insights with the efficiency of multiplexing. Whether you’re studying alternative splicing, novel transcripts, or RNA modifications, our Revio PacBio platform is the ideal solution for your research. Contact us today to learn more, receive a custom quote, and start your journey toward groundbreaking discoveries.
Gene Omics s.r.o.
Republikánská 1218/6, Lobzy, 312 00 Plzeň
IČ: 22273310
Contacts
info@geneomics.eu
+420 725 137 589
