Kinnex single cell RNA
Our Kinnex Single-Cell RNA Sequencing service leverages the advanced Revio PacBio platform to deliver highly accurate, full-length RNA transcripts from individual cells.
This cutting-edge technology provides unparalleled insights into gene expression, isoform diversity, and RNA modifications at the single-cell level. With HiFi sequencing, we enable researchers to uncover the complexities of cellular heterogeneity and functional genomics with unmatched precision and depth.
Advantages of Kinnex Single-Cell RNA Sequencing
- High Accuracy: HiFi sequencing ensures confident detection of transcript variants, even in low-abundance transcripts.
- Full-Length Transcripts: Analyze complete RNA molecules, capturing all isoforms, UTRs, and regulatory elements.
- Single-Cell Sensitivity: Resolve heterogeneity and identify rare cell types or states in mixed populations.
- Scalable Throughput: Process thousands of cells in a single experiment for large-scale studies.
- Epigenetic Insights: Detect RNA modifications and secondary structures directly with minimal bias.
Key Features of Kinnex Single-Cell RNA Sequencing
Full-Length RNA Coverage:
Capture complete transcripts, including splice isoforms, untranslated regions (UTRs), and polyadenylation sites, without fragmentation or assembly.
HiFi Accuracy:
Achieve >99.9% accuracy for confident detection of transcript variants and low-abundance transcripts.
Direct RNA Analysis:
Minimize amplification bias and retain native RNA sequence and epigenetic modifications.
Single-Cell Resolution:
Resolve cellular heterogeneity by profiling thousands of individual cells in a single experiment.
Broad Transcriptome Insights:
Detect novel isoforms, gene fusions, and alternative splicing events with high sensitivity.
Applications of Kinnex Single-Cell RNA Sequencing
Cellular Heterogeneity
Benefits: Identify cell types and states within complex tissues or populations.
Example Use Cases: Tumor microenvironment profiling, immune cell characterization, and stem cell differentiation studies.
Alternative Splicing
Benefits: Detect and quantify splice isoforms at single-cell resolution.
Example Use Cases: Functional annotation of genes and isoform-specific biomarker discovery.
Developmental Biology
Benefits: Trace lineage relationships and gene regulatory networks in single cells.
Example Use Cases: Early embryonic development studies and tissue-specific gene expression profiling.
Disease Research
Benefits: Discover transcriptomic changes associated with diseases at the single-cell level.
Example Use Cases: Cancer research, neurodegenerative disorders, and infectious disease studies.
Gene Editing Validation
Benefits: Confirm transcript-level outcomes of CRISPR/Cas9 or other gene-editing technologies.
Example Use Cases: Verification of on- and off-target effects at the single-cell level.
RNA Input Requirements for Kinnex Single-Cell RNA Sequencing
High-quality RNA is essential for successful single-cell sequencing. The following table outlines the input requirements based on the type of single-cell experiment and transcript length:
Single Cells
Transcript Length: 500 bp–5 kb
Required Input Amount: ≥ 50 cells or equivalent RNA
Purity (RIN): RIN ≥ 7 (intact RNA)
Recommended QC Metrics: Intact RNA with minimal degradation.
Notes: Single-cell RNA extraction or sorted cells preferred for optimal results.
Low-Abundance RNA
Transcript Length: 500 bp–3 kb
Required Input Amount: ≥ 10 ng RNA
Purity (RIN): RIN ≥ 7
Recommended QC Metrics: Clear RNA peaks in electropherogram.
Notes: Suitable for rare cell populations or small sample sizes.
Long Transcripts
Transcript Length: >5 kb
Required Input Amount: ≥ 100 ng RNA
Purity (RIN): RIN ≥ 8
Recommended QC Metrics: High-molecular-weight RNA without degradation.
Notes: For detecting long non-coding RNAs, structural RNAs, or extended UTRs.
Pooled Cells
Transcript Length: Variable
Required Input Amount: ≥ 50–100 cells or ≥ 50 ng RNA
Purity (RIN): RIN ≥ 7
Recommended QC Metrics: Even cell distribution recommended.
Notes: Ideal for bulk-like single-cell RNA sequencing.
Workflow Overview
Sample Preparation & QC
- Extract RNA from sorted cells.
- Perform quality control to ensure RNA integrity and concentration meet requirements.
cDNA Synthesis
- Reverse-transcription of RNA into full-length cDNA using specialized single-cell workflows.
Library Preparation
- HiFi library construction optimized for single-cell RNA applications, ensuring accurate representation of transcripts.
Revio PacBio Sequencing
- Generate long, highly accurate reads to capture full-length RNA molecules from individual cells.
Data Analysis
- Cell clustering and classification.
- Isoform detection and quantification.
- Alternative splicing and gene fusion analysis.
- Functional annotations and pathway analysis.
Get Started with Us
Our Kinnex Single-Cell RNA Sequencing service provides researchers with comprehensive, high-resolution insights into gene expression and transcriptome complexity at the single-cell level. Contact us today to discuss your project goals and learn how the Revio PacBio platform can enhance your research. Let us partner with you to uncover the secrets of cellular diversity and gene regulation!
Gene Omics s.r.o.
Republikánská 1218/6, Lobzy, 312 00 Plzeň
IČ: 22273310
Contacts
info@geneomics.eu
+420 725 137 589
