Kinnex full length RNA
Our Kinnex Full-Length RNA Sequencing service, powered by the Revio PacBio platform, offers precise and comprehensive insights into the transcriptome.
By capturing full-length RNA molecules without fragmentation or assembly, this service enables the detection of splice isoforms, gene fusions, untranslated regions (UTRs), and RNA modifications. With HiFi sequencing technology, researchers gain unmatched accuracy and resolution for transcriptomic analysis across various biological systems.
Advantages of Kinnex Full-Length RNA Sequencing
- High Accuracy: HiFi reads deliver confident isoform identification and quantification.
- Full-Length Transcripts: Avoid incomplete or fragmented reads, ensuring comprehensive transcript coverage.
- Epigenetic Insights: Study RNA modifications and their roles in gene regulation and stability.
- Scalable Solutions: Suitable for small projects or large-scale transcriptomic studies.
- Comprehensive Support: From sample QC to advanced bioinformatics, we provide end-to-end assistance for your research.
Key Features of Kinnex Full-Length RNA Sequencing
Complete Transcript Coverage:
Capture full-length RNA molecules, including complex isoforms, polyadenylation sites, and extended UTRs.
HiFi Read Accuracy:
Achieve >99.9% accuracy for confident isoform detection, even in low-abundance transcripts.
No Fragmentation or Assembly:
Eliminate biases introduced by short-read technologies, preserving native transcript structures.
Epigenetic Insights:
Simultaneously detect RNA modifications, such as m6A methylation, for functional and regulatory analysis.
Broad Transcriptome Insights:
Identify novel transcripts, alternative splicing events, and gene fusions with high sensitivity.
Applications of Kinnex Full-Length RNA Sequencing
Isoform Discovery
Benefits: Comprehensive detection and quantification of alternative splicing events.
Example Use Cases: Annotation of novel isoforms in poorly characterized genomes.
Transcriptome Profiling
Benefits: Quantitative and qualitative insights into the complete transcriptome.
Example Use Cases: Tissue-specific gene expression analysis and biomarker discovery.
Gene Fusion Detection
Benefits: Accurate identification of fusion transcripts in cancer and other diseases.
Example Use Cases: Oncogene discovery and disease-specific RNA signatures.
Epigenetics & Modifications
Benefits: Detect RNA modifications to study transcript stability and translation efficiency.
Example Use Cases: Analysis of regulatory roles of RNA modifications, such as m6A, m5C, and pseudouridine.
Non-Coding RNA Analysis
Benefits: Characterization of long non-coding RNAs (lncRNAs) and circular RNAs.
Example Use Cases: Functional annotation of regulatory RNAs in gene expression and development.
RNA Input Requirements for Kinnex Full-Length RNA Sequencing
The quality and quantity of RNA are critical for successful full-length RNA sequencing. The following table summarizes input requirements based on transcript length:
Short (<1 kb)
Required Input Amount: ≥ 50 ng RNA
Purity (RIN): RIN ≥ 7
Recommended QC Metrics: Clear RNA peaks in electropherogram.
Notes: Suitable for smaller mRNAs and regulatory RNAs.
Medium (1–5 kb)
Required Input Amount: ≥ 100 ng RNA
Purity (RIN): RIN ≥ 8
Recommended QC Metrics: Intact RNA with minimal degradation.
Notes: Ideal for standard transcriptome profiling.
Long (5–10 kb)
Required Input Amount: ≥ 200 ng RNA
Purity (RIN): RIN ≥ 8
Recommended QC Metrics: High-molecular-weight RNA with clear 28S/18S peaks.
Notes: Best for lncRNAs, circular RNAs, and extended transcripts.
Very Long (>10 kb)
Required Input Amount: ≥ 500 ng RNA
Purity (RIN): RIN ≥ 9
Recommended QC Metrics: Intact RNA with no visible degradation.
Notes: Required for extremely large transcripts, such as certain viral or plant RNAs.
Workflow Overview
Sample Preparation & QC
- Perform quality control to confirm RNA integrity, purity, and concentration.
cDNA Synthesis
- Reverse-transcription of RNA into full-length cDNA using PacBio-specific protocols for HiFi library preparation.
Library Preparation
- HiFi library construction optimized for capturing full-length RNA molecules, including rare and long transcripts.
Revio PacBio Sequencing
- Generate highly accurate long reads, capturing complete transcripts without gaps or assembly requirements.
Data Analysis
- Isoform Analysis: Identify and quantify splice isoforms and novel transcripts.
- Gene Expression Profiling: Analyze differential expression across samples or conditions.
- RNA Modifications: Detect RNA modifications and assess their functional impact.
- Functional Annotations: Integrate transcriptomic data with pathway and gene ontology analyses.
Get Started with Kinnex Full-Length RNA Sequencing
Unlock the full potential of transcriptomic research with our Kinnex Full-Length RNA Sequencing service. Whether you’re studying alternative splicing, novel isoforms, or RNA modifications, our Revio PacBio platform provides the precision and depth you need. Contact us today to discuss your project requirements, receive a customized quote, and explore how we can accelerate your discoveries.
Gene Omics s.r.o.
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Contacts
info@geneomics.eu
+420 725 137 589
