Kinnex 16S and RNA amplicons
Our Kinnex 16S and RNA Amplicon Sequencing service, powered by the advanced Revio PacBio platform, offers unparalleled accuracy and long-read capabilities for microbial diversity analysis and RNA studies.
With HiFi sequencing technology, this service is optimized for full-length 16S rRNA gene profiling, ITS analysis, and RNA amplicon sequencing, providing insights into taxonomy, functional potential, and gene expression with unmatched precision.
Advantages of Kinnex 16S and RNA Amplicon Sequencing
- Accuracy: HiFi reads deliver industry-leading accuracy for taxonomic and functional profiling.
- Full-Length Sequencing: Analyze entire 16S rRNA or ITS genes and RNA transcripts, capturing all variants and isoforms.
- Broad Applications: Suitable for microbial community analysis, RNA research, and more.
- Flexible Options: Customize amplicon targets and experimental design to suit your research needs.
- End-to-End Support: From sample QC to advanced bioinformatics, we provide comprehensive support.
Key Features of Kinnex 16S and RNA Amplicon Sequencing
HiFi Read Accuracy:
Achieve >99.9% accuracy, ensuring precise taxonomic classification and variant identification.
Full-Length Amplicon Coverage:
Sequence entire 16S rRNA genes, ITS regions, or RNA amplicons up to 10 kb, eliminating the need for assembly.
Scalable Throughput:
Process hundreds to thousands of amplicons in a single run, enabling high-resolution analysis for complex communities.
Unbiased RNA Analysis:
Accurately quantify RNA variants, splice isoforms, or other transcripts without amplification bias.
Comprehensive Data Outputs:
Taxonomic classification, diversity indices, and functional annotations for microbial studies or RNA profiling.
Applications of Kinnex 16S and RNA Amplicon Sequencing
Microbial Diversity
Benefits: High-resolution taxonomic profiling and diversity analysis using full-length 16S or ITS genes.
Example Use Cases: Gut microbiome analysis, soil and environmental studies, and industrial microbiology.
Pathogen Identification
Benefits: Accurate identification and profiling of pathogenic microbes.
Example Use Cases: Clinical diagnostics, food safety, and epidemiological studies.
Functional Genomics
Benefits: Full-length RNA amplicon sequencing for splice variant and isoform detection.
Example Use Cases: Transcriptomic profiling of eukaryotes or microbial communities.
Agricultural Genomics
Benefits: Microbial community analysis in soil, plants, and livestock.
Example Use Cases: Crop improvement, soil health monitoring, and animal microbiome research.
Industrial Applications
Benefits: Characterization of microbial consortia for bioprocesses.
Example Use Cases: Biofuel production, wastewater treatment, and fermentation studies.
DNA and RNA Input Requirements for Kinnex 16S and RNA Amplicon Sequencing
Proper sample preparation ensures high-quality sequencing results. The table below summarizes the input requirements for DNA and RNA amplicon sequencing based on amplicon length:
16S rRNA
Amplicon Length: 1–2 kb
Required Input Amount: ≥ 50 ng DNA
Purity (A260/A280): 1.8–2.0
Recommended QC Metrics: Clear band on gel electrophoresis; minimal smearing.
Notes: Full-length 16S rRNA sequencing enables high-resolution taxonomic profiling.
ITS Regions
Amplicon Length: 200 bp–1 kb
Required Input Amount: ≥ 50 ng DNA
Purity (A260/A280): 1.8–2.0
Recommended QC Metrics: High-molecular-weight DNA preferred.
Notes: Ideal for fungal diversity and phylogenetic studies.
RNA Amplicons
Amplicon Length: 500 bp–5 kb
Required Input Amount: ≥ 100 ng RNA (cDNA derived)
Purity (A260/A280): 1.8–2.1 (RNA)
Recommended QC Metrics: Intact RNA with RIN > 7 preferred.
Notes: Optimized for full-length transcript sequencing, including splice isoforms.
Long Amplicons
Amplicon Length: 5–10 kb
Required Input Amount: ≥ 200 ng DNA or RNA
Purity (A260/A280): 1.8–2.0 (DNA), 1.8–2.1 (RNA)
Recommended QC Metrics: High-molecular-weight, intact DNA/RNA required.
Notes: Best for complex microbial communities or long RNA isoform analysis.
Workflow Overview
Sample Preparation & QC
- DNA and RNA samples are assessed for concentration, purity, and integrity.
- High-quality RNA is reverse-transcribed into cDNA for sequencing.
Amplicon Design & Enrichment
- Custom primers are designed for 16S rRNA, ITS, or RNA regions of interest.
- Enrichment steps ensure specific and unbiased amplification of target regions.
Library Preparation
- HiFi library preparation optimized for long-read sequencing minimizes errors and bias.
Sequencing
- The Revio PacBio platform generates highly accurate long reads, covering entire amplicons.
Data Analysis
- 16S/ITS Analysis: Taxonomic classification, alpha/beta diversity metrics, and functional predictions.
- RNA Amplicon Analysis: Transcript quantification, splice isoform detection, and variant analysis.
Get Started with Us
Our Kinnex 16S and RNA Amplicon Sequencing service enables groundbreaking research in microbial ecology, functional genomics, and RNA biology. Contact us today to discuss your project requirements and receive a personalized consultation. Let us help you unlock the full potential of the Revio PacBio platform for your sequencing needs!
Would you like additional details on specific applications, bioinformatics pipelines, or case studies?
Gene Omics s.r.o.
Republikánská 1218/6, Lobzy, 312 00 Plzeň
IČ: 22273310
Contacts
info@geneomics.eu
+420 725 137 589
