Amplicons sequencing
Our Amplicon Sequencing service using the state-of-the-art Revio PacBio platform offers unparalleled accuracy, long-read capability, and detailed resolution of targeted DNA regions.
This service is ideal for applications such as variant detection, microbial diversity studies, and the analysis of complex genomic loci, providing high-quality insights for research and clinical applications.
Whether you’re studying microbial communities, investigating genetic variants, or analyzing specific genomic regions, our Revio PacBio Amplicon Sequencing service provides reliable, high-quality results. Contact us today for a consultation and personalized quote tailored to your research needs.
Key Features of Revio PacBio Amplicon Sequencing
High Accuracy with HiFi Reads:
Achieve >99.9% accuracy, ensuring reliable results for amplicon sequencing, even in challenging regions.
Long-Read Capability:
Sequence amplicons up to 10 kb or more, allowing full-length analysis of targeted regions without assembly.
Scalable Throughput:
Process hundreds to thousands of amplicons in a single run, making it suitable for small and large-scale studies.
Comprehensive Data:
Detect SNPs, indels, structural variations, and epigenetic modifications with precision.
Minimal Amplification Bias:
High-quality library preparation reduces PCR-induced errors and ensures even representation of amplicons.
Applications of Amplicon Sequencing on Revio PacBio
Human Genomics
Benefits: Detection of rare variants, structural variations, and expanded genomic regions.
Example Use Cases: Inherited disease studies, cancer genomics, pharmacogenomics.
Plant Genomics
Benefits: Resolving complex genomes with polyploidy and high heterozygosity.
Example Use Cases: Crop improvement, trait discovery, and stress tolerance research.
Animal Genomics
Benefits: Structural variant detection and high-quality genome assemblies for livestock and wildlife.
Example Use Cases: Animal breeding, conservation genomics, and health research.
Microbial Genomics
Benefits: Complete microbial genome assembly and diversity analysis.
Example Use Cases: Pathogen characterization, strain typing, and antibiotic resistance studies.
Metagenomics
Benefits: High-resolution taxonomic profiling and functional analysis of mixed microbial communities.
Example Use Cases: Gut microbiome research, environmental studies, and industrial microbiology.
De Novo Assembly
Benefits: Construction of highly contiguous, reference-grade genome assemblies.
Example Use Cases: Assembling genomes of novel species, improving fragmented assemblies, and evolutionary studies.
DNA Input Requirements for Amplicon Sequencing
The quality and quantity of input DNA are critical for successful amplicon sequencing. Below is a table summarizing DNA input requirements based on amplicon length and sample type:
Plants
Required DNA Amount: 5–10 µg
Purity (A260/A280): 1.8–2.0
Integrity: High-molecular-weight DNA (>20 kb); minimal degradation.
Key Challenges: High polysaccharide and phenolic contamination.
Animals
Required DNA Amount: 2–5 µg
Purity (A260/A280): 1.8–2.0
Integrity: Intact DNA; minimal fragmentation.
Key Challenges: Tissue-specific variations; some tissues may yield degraded DNA.
Microbes
Required DNA Amount: 1–2 µg
Purity (A260/A280): 1.8–2.0
Integrity: High-molecular-weight DNA (>15 kb) for accurate assemblies.
Key Challenges: Extraction challenges with tough cell walls (e.g., Gram-positive bacteria).
Metagenomics
Required DNA Amount: 5–10 ng (per organism)
Purity (A260/A280): 1.8–2.0
Integrity: Even representation of DNA from mixed samples; high integrity.
Key Challenges: Low-abundance DNA, contamination by host DNA, and bias during extraction.
De Novo Assembly
Required DNA Amount: 5–20 µg (depending on genome size)
Purity (A260/A280): 1.8–2.0
Integrity: Ultra-high-molecular-weight DNA (>50 kb preferred).
Key Challenges: Handling large, complex genomes with repetitive regions and high heterozygosity.
Advantages of Amplicon Sequencing with Revio PacBio
- Accuracy: HiFi reads eliminate errors common in other sequencing technologies, ensuring high-confidence variant detection.
- Full-Length Analysis: Long-read capability enables sequencing of entire amplicons, capturing complete variants and structural features.
- Cost-Effective: Scalable throughput reduces the per-sample cost, making it ideal for large projects.
- Flexibility: Suitable for a wide range of applications, from microbial diversity to gene editing validation.
Workflow Overview
Sample Preparation & QC
Input DNA is assessed for concentration, purity, and integrity to ensure high-quality sequencing data.
Amplicon Design
Assistance with primer design and optimization for specific genomic regions of interest.
Library Preparation
High-fidelity library preparation optimized for the Revio PacBio platform, ensuring even representation of all amplicons.
Sequencing
High-throughput sequencing generates HiFi reads with unmatched accuracy.
Data Analysis
Comprehensive bioinformatics services tailored to amplicon sequencing, including:
- Variant calling (SNPs, indels, and SVs)
- Taxonomic and phylogenetic profiling (for microbial studies)
- Functional annotation and interpretation
Gene Omics s.r.o.
Republikánská 1218/6, Lobzy, 312 00 Plzeň
IČ: 22273310
Contacts
info@geneomics.eu
+420 725 137 589
